Effects of angiotensin Ⅱ on connexin 43 of VSMCs in arteriosclerosis     

CAI Wei RUAN Li-ming WANG Yi-na CHEN Jun-zhu 《Journal of Zhejiang University. Science. B》2006,7(8):648-653

Objective： To observe the effect ofangiotensin Ⅱ （Ang Ⅱ） on expression of gap junction channel protein connexin 43 （Cx43） in the proliferation process of vascular smooth muscle cells （VSMCs） during the early stage of arteriosclerosis. Methods： Thirty-two adult male rabbits were randomly divided into 4 groups. Rabbits in Group A were fed common diet while others in Groups B, C, and D were fed high-cholesterol diet. Losartan （10 mg/（kg.d）） and ramipril （0.5 mg/（kg-d）） were added in the diet of Groups C and D, respectively. The animals were sacrificed after 8 weeks and abdominal aortas were removed and dissected. The expression of Cx43 was assayed using RT-PCR and Western Blotting analysis. Results： Cx43 was increased markedly in both protein and mRNA level in Groups B, C, and D fed high-cholesterol diet compared with that in control group （P〈0.01）. Cx43 level in losartan or ramipril treated groups was higher than that in control group （P〈0.01, P〈0.05）, but lower than that in high-cholesterol diet groups （P〈0.05, P〈0.01）. Conclusion： Cx43 level was upregulated in VSMCs during early atherosclerosis. Losartan and ramipril can inhibit the expression of Cx43.  相似文献   

支持向量分类算法在5-HT3受体拮抗剂的构效关系研究中的应用     

杨善升  陆文聪  纪晓波  陈念贻 《上海大学学报(英文版)》2006,10(4):366-370

In this work, support vector classification （SVC） algorithm was used to build structure-activity relationship （SAR） model of the 5-hydroxytryptamine type 3 （5-HT3 ） receptor antagonists with 26 compounds. In a benchmark test, SVC was compared with several techniques of machine learning currently used in the field. The prediction performance of the model was discussed on the basis of the leave-one-out cross-validation. The results show that the accuracy of prediction of SVC model was higher than those of back propagation artificial neural network （BP ANN）, K-nearest neighbor （KNN） and Fisher methods.  相似文献   

人体大肠癌雌孕激素受体的研究     

薄爱华  戴洁  邢立强  夏苓  许永利  张振忠  么金满 《河北北方学院学报(医学版)》1994,(1)

我们利用冰冻切片和酶联(E_2—HRP,Pg—HRP)免疫组织化学方法、检测了82例大肠癌组织中的雌、孕激素受体(ER、PR),结果表明:ER阳性率为54.87％、PR阳性率为45.12％。本实验提示在大肠癌45—54％的病例属激素依赖型。文中对ER、PR的结构和作用机理进行了阐述。  相似文献   

应用地高辛标记的cRNA探针原位杂交法检测大鼠下丘脑生长抑素mRNA     

李英  周济远  任君旭  王建伟  白雪梅  张耕 《河北北方学院学报(医学版)》1996,(2)

用成年雄性Wistar大鼠,经4％多聚甲醛灌注固定,取下丘脑组织,行再固定24h,常规石蜡包埋和切片,用地高辛标记的cRNA探针进行原位杂交,结果显示在弓状核及其邻近区域见到阳性神经元,阳性信号为蓝色颗粒,胞质、核膜及核仁呈阳性。阴性对照均无阳性反应出现。本实验证实,用石蜡切片进行原位杂交组织化学研究,能检测下丘脑生长抑素神经元基因表达产物mRNA,方法稳定,重复性好,便于普通实验室开展工作。  相似文献   

Characteristics of transgenic tomatoes antisensed for the ethylene receptor genes LeERT1 and LeERT2     

Wang ZF  Ying TJ  Zhang Y  Bao BL  Huang XD 《Journal of Zhejiang University. Science. B》2006,7(7):591-595

Two stable transformed lines containing antisense LeERT1 or LeERT2 sequences and their hybridized line were investigated to determine the effect of LeERT1 and LeERT2 specificity in the ethylene receptor family in tomato （Lycopersicon esculenturn Mill.） on ethylene signaling. The transgenic line alel containing antisense LeER T1 displayed shorter length of seedling grown in the dark and adult plant in the light, severe epinastic petiole, and accelerated abscission of petiole explant and senescence of flower explant, compared with its wild type B 1. The transgenic line ale2 containing antisense LeERT2 also exhibited shorter hypocotyls and slightly accelerated abscission. The phenotypes of cross line dale of LeERT1 and LeERT2 were close to alel in many aspects. These results suggested that LeERT1 probably plays a relatively important role in ethylene signaling of tomato growth and development.  相似文献   

The diagnostic significance of the detection of cytokeratin 19 mRNA by quantitative RT-PCR in benign and malignant pleural effusions     

徐峰  陈杰  沈华浩  王选锭  单江 《Journal of Zhejiang University. Science. B》2004,(10)

  相似文献   

The diagnostic significance of the detection of cytokeratin 19 mRNA by quantitative RT-PCR in benign and malignant pleural effusions     

徐峰  陈杰  沈华浩  王选锭  单江 《浙江大学学报(A卷英文版)》2004,5(10):1286-1289

  相似文献   

miRNA:一种新的基因调控元件     

张西玉 《乐山师范学院学报》2006,21(12):51-53

miRNA(microRNA)是一类长约22个核苷酸的小分子非编码RNA。近年来,科学家发现miRNA与基因表达调控密切相关,miRNA可通过与靶mRNA特异性的碱基配对,引起靶mRNA的降解,抑制其翻译,或以其它形式的调节机制抑制靶基因的表达。本文概述了miRNA特点、生物合成过程、功能以及作用机制。  相似文献   

Study of Resistin gene expression in peripheral blood mononuclear cell and its gene polymorphism in a small range population     

Yang YM  Xu ZR  Wu LJ  Huang WD 《Journal of Zhejiang University. Science. B》2007,8(2):132-135

INTRODUCTION Recently discovered as an endocrine organ, adi-pose tissue can secrete many cytokines such as Re-sistin, adiponectin and free fatty acids, and receives much attention in the study of insulin resistance and type 2 diabetes mellitus (T2DM). As a peptide hor-mone secreted by adipocyte, Resistin is considered to be the linkage between obesity and insulin resistance (Steppan et al., 2001). Most studies on Resistin in-vestigate adipose tissue. In this experiment we de-tected t…  相似文献   

以氢键和静电作用的阴离子识别受体的研究进展     

林珍 《忻州师范学院学报》2007,23(2):37-42

总结了近年来以氢键和静电作用的阴离子荧光受体的研究进展,主要介绍以下4类受体的设计合成及其在阴离子识别中的应用:(1)含多酰胺基荧光受体;(2)含脲、硫脲基的荧光受体;(3)含胍盐和硫脲盐的荧光受体;(4)含五元杂环的荧光受体。  相似文献